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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
10/01/2005 |
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Data da última atualização: |
03/08/2017 |
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Autoria: |
GRANGE, L.; HUNGRIA, M. |
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Título: |
Genetic diversity of indigenous common bean (Phaseolus vulgaris) rhizobia in two Brazilian ecosystems. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Soil Biology and Biochemistry, Oxford, v. 36, n. 9, p. 1389-1398, Sept. 2004. |
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Idioma: |
Inglês |
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Conteúdo: |
Although rhizobia for common bean (Phaseolus vulgaris L.) are established in most Brazilian soils, understanding of their genetic diversity is very poor. This study characterized bean strains from two contrasting ecosystems in Brazil, the Northeast Region, with a semi-arid climate and neutral soils and the South Region, with a humid subtropical climate and acid soils. Seedlings of the cultivars Negro Argel and Aporé were used to trap 243 rhizobial isolates from 12 out of 14 sites. An analysis of ERIC-PCR products revealed enormous variability, with 81% of the isolates representing unique strains considering a level of 70% of similarity. In general, there was no effect of either the bean cultivar, or the ecosystem on rhizobial diversity. One-hundred and one strains showing genetic relatedness (ERIC-PCR) less than 70% were further analyzed using restriction fragment length polymorphism (RFLP) of the 16 S rDNA cleaved with five restriction enzymes. Twenty-five different profile combinations were obtained. Rhizobium etli was the predominant species, with 73 strains showing similar RFLP profiles, while 12 other strains differed only by the profile with one restriction enzyme. Fifty strains were submitted to sequencing of a 16 S rDNA fragment, and 34 clustered with R. etli, including strains with RFLP-PCR profiles similar to those species or differing by one restriction enzyme. However, other strains differing by one or two enzymes were genetically distant from R. etli and two strains with identical profiles showed higher similarity to Sinorhizobium fredii. Other strains showed higher similarity of bases with R. tropici, R. leguminosarum and Mesorhizobium plurifarium, but some strains were quite dissimilar and may represent new species. Great variability was also verified among the sequenced strains in relation to the ability to grow in YMA at 40 °C, in LB, to synthesize melanin in vitro, as well as in symbiotic performance, including differences in relation to the described species, e.g. many R. etli strains were able to grow in LB and in YMA at 40 °C, and not all R. tropici were able to nodulate Leucaena. MenosAlthough rhizobia for common bean (Phaseolus vulgaris L.) are established in most Brazilian soils, understanding of their genetic diversity is very poor. This study characterized bean strains from two contrasting ecosystems in Brazil, the Northeast Region, with a semi-arid climate and neutral soils and the South Region, with a humid subtropical climate and acid soils. Seedlings of the cultivars Negro Argel and Aporé were used to trap 243 rhizobial isolates from 12 out of 14 sites. An analysis of ERIC-PCR products revealed enormous variability, with 81% of the isolates representing unique strains considering a level of 70% of similarity. In general, there was no effect of either the bean cultivar, or the ecosystem on rhizobial diversity. One-hundred and one strains showing genetic relatedness (ERIC-PCR) less than 70% were further analyzed using restriction fragment length polymorphism (RFLP) of the 16 S rDNA cleaved with five restriction enzymes. Twenty-five different profile combinations were obtained. Rhizobium etli was the predominant species, with 73 strains showing similar RFLP profiles, while 12 other strains differed only by the profile with one restriction enzyme. Fifty strains were submitted to sequencing of a 16 S rDNA fragment, and 34 clustered with R. etli, including strains with RFLP-PCR profiles similar to those species or differing by one restriction enzyme. However, other strains differing by one or two enzymes were genetically distant from R. etli and two str... Mostrar Tudo |
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Palavras-Chave: |
Rizóbio. |
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Thesagro: |
Feijão. |
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Categoria do assunto: |
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Marc: |
LEADER 02618naa a2200157 a 4500
001 1467760
005 2017-08-03
008 2004 bl --- 0-- u #d
100 1 $aGRANGE, L.
245 $aGenetic diversity of indigenous common bean (Phaseolus vulgaris) rhizobia in two Brazilian ecosystems.
260 $c2004
520 $aAlthough rhizobia for common bean (Phaseolus vulgaris L.) are established in most Brazilian soils, understanding of their genetic diversity is very poor. This study characterized bean strains from two contrasting ecosystems in Brazil, the Northeast Region, with a semi-arid climate and neutral soils and the South Region, with a humid subtropical climate and acid soils. Seedlings of the cultivars Negro Argel and Aporé were used to trap 243 rhizobial isolates from 12 out of 14 sites. An analysis of ERIC-PCR products revealed enormous variability, with 81% of the isolates representing unique strains considering a level of 70% of similarity. In general, there was no effect of either the bean cultivar, or the ecosystem on rhizobial diversity. One-hundred and one strains showing genetic relatedness (ERIC-PCR) less than 70% were further analyzed using restriction fragment length polymorphism (RFLP) of the 16 S rDNA cleaved with five restriction enzymes. Twenty-five different profile combinations were obtained. Rhizobium etli was the predominant species, with 73 strains showing similar RFLP profiles, while 12 other strains differed only by the profile with one restriction enzyme. Fifty strains were submitted to sequencing of a 16 S rDNA fragment, and 34 clustered with R. etli, including strains with RFLP-PCR profiles similar to those species or differing by one restriction enzyme. However, other strains differing by one or two enzymes were genetically distant from R. etli and two strains with identical profiles showed higher similarity to Sinorhizobium fredii. Other strains showed higher similarity of bases with R. tropici, R. leguminosarum and Mesorhizobium plurifarium, but some strains were quite dissimilar and may represent new species. Great variability was also verified among the sequenced strains in relation to the ability to grow in YMA at 40 °C, in LB, to synthesize melanin in vitro, as well as in symbiotic performance, including differences in relation to the described species, e.g. many R. etli strains were able to grow in LB and in YMA at 40 °C, and not all R. tropici were able to nodulate Leucaena.
650 $aFeijão
653 $aRizóbio
700 1 $aHUNGRIA, M.
773 $tSoil Biology and Biochemistry, Oxford$gv. 36, n. 9, p. 1389-1398, Sept. 2004.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 22 | |
| 5. |  | GRANGE, L.; HUNGRIA, M. Diversidade de rizobio capaz de nodular o feijoeiro isolado de solos da Regiao Nordeste e da Regiao Sul do Brasil. Genetics and Molecular Biology, Ribeirao Preto, v.23, n.3, p.353-354, Sept. 2000. Supplement. Trabalho apresentado no 46, Congresso Nacional de Genetica, 19 a 23 de setembro de 2000, Aguas de Lindoia, SP.| Biblioteca(s): Embrapa Soja. |
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| 6. | | NAVROSKI, D.; COLOZZI FILHO, A.; MOREIRA, A.; GRANGE, L. Atividade da enzima fosfatase ácida em diferentes manejos de solo na região oeste do Paraná. In: REUNIÃO BRASILEIRA DE MANEJO E CONSERVAÇÃO DO SOLO E DA ÁGUA, 20., 2016, Foz do Iguaçu. O solo sob ameaça: conexões necessárias ao manejo e conservação do solo e água: anais. Curitiba: SBCS-NEPAR; Londrina: IAPAR, 2016. p. 738-740. 1 Livro digital. Editado por Arnaldo Colozzi Filho, João Henrique Caviglione, Graziela Moraes de Cesare Barbosa, Luciano Grillo Gil, Tiago Santos Telles.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 9. | | FERREIRA, M. C.; GUERRA, B.; GRANGE, L.; CHUEIRE, L. M. O.; MENDES, I. C.; HUNGRIA, M. Metodos moleculares para a avaliacao de estirpes de rhizobio em solos brasileiros. In: CONGRESSO BRASILEIRO DE CIENCIA DO SOLO, 28., 2001, Londrina. Ciencia do solo: fator de produtividade e competitividade com sustentabilidade. Londrina: Sociedade Brasileira de Ciencia do Solo, 2001. p.79.| Biblioteca(s): Embrapa Soja. |
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| 10. | | HUNGRIA, M.; CAMPO, R. J.; CHUEIRE, L. M. O.; GRANGE, L.; MEGIAS, M. Symbiotic effectiveness of fast-growing rhizobial strains isolated from soybean nodules in Brazil. Biology and Fertility of Soils, Berlin, v. 33, n. 5, p. 387-394, 2001.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - B |
| Biblioteca(s): Embrapa Soja. |
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| 11. | | HUNGRIA, M.; CHUEIRE, L. M. O.; GRANGE, L.; LAMBRET, Y.; MEGIAS, M. Symbiotic performance of soybean sinorhizobia strains isolated in Brazil. In: INTERNATIONAL CONGRESS ON NITROGEN FIXATION, 12., 1999, Foz do Iguacu. Nitrogen fixation: from molecules to crop productivity: proceedings. Dordrechet: Kluwer, 2000. p. 625. (Current Plant Science and Biotechnology in Agriculture, 38). Editado por Fabio O. Pedrosa, Mariangela Hungria, Geoffrey Yates, William E. Newton.| Biblioteca(s): Embrapa Soja. |
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| 12. | | HUNGRIA, M.; GRANGE, L.; MENDES, I. C.; ANDRADE, D. S.; CHUEIRE, L. M. O.; CAMPO, R. J. Diversity of bean (Pahseolus vulgaris L.) rhizobia in Brazil. In: SIMPOSIO DE RECURSOS GENETICOS PARA A AMERICA LATINA E CARIBE - SIRGEALC, 3., 2001. Londrina, PR. Recursos geneticos: conservar para a vida: anais. Londrina: IAPAR; Brasilia: Embrapa Recursos Geneticos e Biotecnologia, 2001. p. 487-489. Eventos paralelos: III. Reuniao Latino Americana de Especialistas em Arachis; III. Reuniao Latino Americana de Especialistas em Recursos Geneticos Florestais.| Biblioteca(s): Embrapa Cerrados. |
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| 13. | | HUNGRIA, M.; GRANGE, L.; MENDES, I. C.; ANDRADE, D. S.; CHUEIRE, L. M. O.; CAMPO, R. J. Diversity of bean (Phaseolus vulgaris L.) rhizobia in Brazil. In: SIMPÓSIO DE RECURSOS GENÉTICOS PARA A AMÉRICA LATINA E CARIBE, 3.; REUNIÃO LATINO AMERICANA DE ESPECIALISTAS EM ARACHIS, 3.; REUNIÃO LATINO AMERICANA DE ESPECIALISTAS EM RECURSOS GENÉTICOS FLORESTAIS, 3., 2001, Londrina. Anais..Londrina: IAPAR, 2001. p. 487-489. III SIRGEALC| Biblioteca(s): Embrapa Soja. |
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| 14. | | HUNGRIA, M.; CAMPO, R. J.; ANDRADE, D. S.; CHUEIRE, L. M. de O.; GRANGE, L.; FERREIRA, M. C. Diversity of symbiotic nitrogen-fixing bacteria in brazilian soils. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 21., 2001, Foz do Iguaçu-PR. Resumos... Foz do Iguaçu: Sociedade Brasileira de Microbiologia, 2001. Trab. MS-037.| Biblioteca(s): Embrapa Soja. |
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| 15. | | HUNGRIA, M.; CAMPO, R. J.; CHUEIRE, L. M. O.; MENDES, I. C.; FRANCHINI, J. C.; ANDRADE, D. S.; NICOLÁS, M. F.; GRANGE, L.; LOUREIRO, M. de F. Aspectos agronômicos da fixação simbiótica do nitrogênio no Brazil. In: REUNIÓN LATINOAMERICANA DE RHIZOBIOLOGIA, 21.; CONGRESO NACIONAL DE LA FIJACIÓN BIOLÓGICA DE NITRÓGENO, 6., 2002. Cocoyoc. Memorias... [S.l.]: Centro de Investigación sobre Fijación de Nitrógeno; Universidade Nacional Autónoma de México, 2002. p. 53.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 16. | | CHUEIRE, L. M. de O.; BANGEL, E.; FERREIRA, M. C.; GRANGE, L.; CAMPO, R. J.; MOSTASSO, F. L.; ANDRADE, D. de S.; PEDROSA, F. O.; HUNGRIA, M. Classificacao taxonomica, baseada na caracterizacao molecular, das estirpes de rizobio recomendadas para as culturas da soja e do feijoeiro. Londrina: Embrapa Soja, 2000. 32 p. (Embrapa Soja. Boletim de Pesquisa, 3).| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Soja. |
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| 17. | | FERREIRA, E. G. C.; GOMES, D. F.; DELAI, C. V.; BARREIROS, M. A. B.; GRANGE, L.; RODRIGUES, E. P.; MERTZ-HENNING, L. M.; BARCELLOS, F. G.; HUNGRIA, M. Revealing potential functions of hypothetical proteins induced by genistein in the symbiosis island of Bradyrhizobium japonicum commercial strain SEMIA 5079 (= CPAC 15). BMC Microbiology, v. 22, 122, 2022. 20 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 18. | | HUNGRIA, M.; CAMPO, R. J.; CHUEIRE, L. M. O.; BANGEL, E. V.; COLOZZI-FILHO, A.; GRANGE, L.; KASCHUK, G.; LOUREIRO, M. de F. Uso de marcadores moleculares para maximizar a contribuição dos rizóbios fixadores de nitrogênio e dos fungos micorrízicos arbusculares: caracterização da diversidade sob diferentes manejos agrícolas e avaliação das respostas à inoculação. In: SARAIVA, O. F. (Org.). Resultados de pesquisa da Embrapa Soja - 2003: microbiologia de solos. Londrina: Embrapa Soja, 2004. p. 59-62. (Embrapa Soja. Documentos, 243).| Biblioteca(s): Embrapa Soja. |
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| 19. | | HUNGRIA, M.; CAMPO, R. J.; ANDRADE, D. S.; MENDES, I. C.; CHUEIRE, L. M. O.; GRANGE, L.; FERREIRA, M. C.; ALBERTON, O.; KASCHUK, G.; LOUREIRO, M. F.; PEDROSA, F. O. Genetic diversity of symbiotic rhizobia in brazilian soils. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUÍMICA E BIOLOGIA MOLECULAR, 31., 2002, Caxambu. Programa e resumos. São Paulo : SBBq, 2002. p.29.| Biblioteca(s): Embrapa Soja. |
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| 20. | | HUNGRIA, M.; CAMPO, R. J.; CHUEIRE, L. M. O.; GRANGE, L.; GERMANO, M. G.; MENNA, P.; BARCELLOS, F. G.; GALLI-TERASAWA, L. V.; PINTO, F. G. S. NICOLAS, M. F.; MENDES, I. C. Rhizobial diversity in brazilian soils: indigenous population, variability due to agronomic management identification of genes related to symbiotic performance. In: LATIN-AMERICAN CONFERENCE ON RHIZOBIOLOGY, 22.; BRAZILIAN CONFERENCE ON BIOLOGICAL NITROGEN FIXATION, 1., 2004, Miguel Pereira. Programme and Abstracts. Seropédica: Embrapa Agrobiologia, 2004. p. 17. Editado por Veronica Massena Reis, Marta Bahia.| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 22 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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